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‘instead of targeting cysteines, target lysines or serine or tyrosine or other amino acid, I think the approach widely used in many areas of biology have. ‘.. In a final series of experiments in principle,gator David Baker and colleagues at the University of Washington delivered a number of synthetic proteins, which was designed to work as enzymes. ‘This is a relatively simple and accurate method for screening designed proteins for functional properties,’said Cravatt. ‘It might be very useful for creating new enzyme catalysts for basic research and industrial applications. ,, in principle, is to change the reactive group on the probe, and ‘said Cravatt.
For more than 90 % of these cysteines, showed low and high concentration of probes corresponding to low and high labeling, indicating that the cysteines had ordinary reactivity. – ‘But a small fraction of of the cysteines showed a constant level of labeling for low and high concentration probes,’said Wang, ‘indicating that they were hyper-reactive. ‘.. ‘to find a to find a more efficient way and to quantitatively analyze reactive sites,’said Cravatt, ‘not just for a protein that a purified sample a purified sample but for a large group of proteins in their natural environment a whole cell a whole cell or a tissue. Continue reading